FIG. 4.

TEX14 interacts with CEP55. (A) Immunoprecipitation (IP) of FLAG-TEX14 and/or MYC-CEP55 from HEK293T cells, followed by Western blot analysis with the antibodies as shown. Immunoprecipitation of protein G (lanes G) is the control. (B) Yeast two-hybrid analyses using vectors encoding full-length mouse TEX14, MKLP1, CEP55, and positive and negative controls. The relative ratios of TEX14-TEX14, TEX14-MKLP1, and TEX14-CEP55 interactions were determined by using an oxygen-biosensor system. (C) Yeast two-hybrid interactions of mouse and human full-length TEX14 and CEP55. (a) Yeast were stably transformed with vectors and plated for yeast two-hybrid analysis as depicted. SV40 T antigen with p53 and SV40 T antigen with lamin C were used as positive and negative controls, respectively. (b) Interactions between the two-hybrid proteins are evident by colony growth and blue color on selection plates. (c) A nonselection plate shows that all of the transformed yeast are capable of growing.