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. 2010 Feb 22;30(9):2280–2292. doi: 10.1128/MCB.01392-09

FIG. 5.

FIG. 5.

The region encoding the conserved TEX14 GPPX3Y motif interacts with the hinge region of CEP55. (A) The full-length and truncated regions of mouse TEX14 and CEP55 were cloned into the yeast two-hybrid vectors and used for the studies in panels B and C below. (B and C) Yeast two-hybrid oxygen biosensor between the full-length and truncated TEX14 proteins and the full-length CEP55 protein and/or selection plate analyses of the full-length and truncated TEX14 and CEP55 proteins. The terms “+” and “-” indicate positive and negative interactions, respectively. (D) Alignment of the GPPX3Y motif and flanking sequences from TEX14 orthologs and human ALIX and TSG101. Conserved amino acids are highlighted.