TABLE 1.
Constructs and results of yeast two-hybrid and biosensor experimentsa
| Assay | Figure | Species | Proteinb |
Interactionc | |
|---|---|---|---|---|---|
| pGADT7 | pGBKT7 | ||||
| Biosensor | 4B | Mouse | TEX14 | TEX14 | ++ |
| Mouse | MKLP1 | TEX14 | + | ||
| Mouse | CEP55 | TEX14 | ++ | ||
| 5B | Mouse | T1 | CEP55 | − | |
| Mouse | T2 | CEP55 | − | ||
| Mouse | T3 | CEP55 | +++ | ||
| Mouse | T1 + T2 | CEP55 | − | ||
| Mouse | T4 | CEP55 | ++ | ||
| Mouse | TEX14 | CEP55 | ++ | ||
| Y2H | 4C | Mouse | TEX14 | TEX14 | ++ |
| Mouse | TEX14 | CEP55 | ++ | ||
| Human | TEX14 | TEX14 | − | ||
| Human | TEX14 | CEP55 | ++ | ||
| 5C | Mouse | TEX14 | CEP55 | ++ | |
| Mouse | T3 | CEP55 | ++ | ||
| Mouse | T4 | CEP55 | ++ | ||
| Mouse | T4-C | CEP55 | − | ||
| Mouse | TEX14 | C1 | ++ | ||
| Mouse | T3 | C1 | ++ | ||
| Mouse | T4 | C1 | ++ | ||
| Mouse | T4-C | C1 | − | ||
a
This table summarizes the results of the biosensor and yeast two-hybrid assays in Fig. 4B and C and 5B and C. The source of the proteins that we used in these experiments is given in the species column.
b
TEX14, CEP55, and MKLP1 indicate full-length proteins, whereas T1 to T4, T4-C, or C1 denotes truncated proteins.
c
The terms “+” and “−” indicate positive and negative interactions, respectively, and the number of “+” symbols indicates the strength of the interaction.