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. 2010 Feb 16;30(9):2090–2104. doi: 10.1128/MCB.01318-09

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FIG. 6. — Stimulating heterochromatin formation on centromeres in CENP-A-depleted TIG3 cells. (A to C) Immunofluorescence of TIG3 cells depleted of CENP-A performed on day 10 after infection. Staining revealed CENP-B (red in overlay) and CENP-A (green in overlay) (A), HP1β (green in overlay) (B), or HP1γ (green in overlay) (C). DNA was counterstained with Hoechst (blue in overlay). In panels B and C, DNA focus-negative (middle panels) and DNA focus-positive cells depleted of CENP-A (lower panels) are shown. Insets represent enlarged images indicated by arrows. Scale bars, 10 μm.