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shRNA target sequences and primers used for qPCR
| Primer | Sequence (5′-3′) |
|---|---|
| Control shRNA | CATTGCTATAGAGGCAGAT |
| CENP-A shRNA | AGGAGATCCGAAAGCTTCA |
| p53 shRNA | GACTCCAGTGGTAATCTAC |
| p16INK4a shRNA | GAGGAGGTGCGGGCGCTGC |
| p21CIP1 qPCR forward | GAGACTCTCAGGGTCGAAAACG |
| p21CIP1 qPCR reverse | GATTAGGGCTTCCTCTTGGAGAA |
| p16INK4a qPCR forward | CATAGATGCCGCGGAAGGT |
| p16INK4a qPCR reverse | AAGTTTCCCGAGGTTTCTCAGA |
| GAPDH qPCR forward | CAGGGCTGCTTTTAACTCTGGTA |
| GAPDH qPCR reverse | AATTTGCCATGGGTGGAATC |
