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. 2010 Mar 1;30(9):2181–2192. doi: 10.1128/MCB.01441-09

FIG. 6.

FIG. 6.

ChIP analysis of Tal1 and E47 occupancy of the p16(Ink4a) and Gata2 genes in wild-type MM precursors cultured for 4 (A) or 7 (B) days. DNA derived from chromatin fragments immunoprecipitated with antibodies to Tal1 or E47 or normal rabbit IgG was quantified by real-time PCR analysis using region-specific primers. Each bar represents the mean abundance ± the standard deviation of each fragment relative to the input as determined from triplicate PCR measurements. ChIP analysis of Tal1 (C) and E2a (D) occupancy of the p16(Ink4a) and Gata2 genes in M1 cells cultured with or without IL-6 for 48 h. DNA derived from chromatin fragments immunoprecipitated with antibodies to Tal1 or E2a or normal rabbit IgG was quantified by real-time PCR analysis using region-specific primers. Each bar represents the mean abundance ± the standard deviation of each fragment relative to the input as determined from triplicate PCR measurements.