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. 2010 Mar 1;30(9):2229–2240. doi: 10.1128/MCB.00723-09

FIG. 2.

FIG. 2.

DKO MEFs display normal MT organization in a 3D context. (A) Immunofluorescence analysis of α-tubulin (FITC; green) and F-actin (phalloidin, red) in WT, p27 KO, and DKO MEFs immersed in a 3D collagen I matrix for 6 h and subjected to the tubulin dilution assay prior to fixation. A typical image acquired by confocal microscopy is shown. (B) Immunofluorescence analysis of acetylated tubulin (green) and F-actin (phalloidin; red) in WT, p27 KO, and DKO MEFs immersed in a 3D collagen I matrix for 6 h. A typical image acquired by confocal microscopy is shown. (C) Immunofluorescence analysis of acetylated tubulin (green) and F-actin (phalloidin; red) in WT, p27 KO, and DKO MEFS alowed to adhere to collagen I for 30 min. Cells were fixed in methanol-acetone to allow outflow of soluble proteins. A typical image acquired by confocal microscopy is shown.