FIG. 5.
Binding of HP-OVA to sCD4 and gp120 as assessed by SPR assay. (A) Dose-dependent binding of HP-OVA to sCD4 (A), to gp120 from HIV-1IIIB (B), and to gp120 from HIV-1BaL (C) was observed. sCD4 or gp120 was immobilized onto a CM3 sensor chip. HP-OVA at various concentrations (0.16 to 5.00 μM) was injected onto the surface for 200 s at a flow rate of 30 μl/min and then washed with HBS-EP. The results from the association and dissociation reactions were determined. The response units were recorded against the flow time (s).