FIG. 6.
Inhibition of gp41 six-helix bundle (6-HB) formation by HP-OVA. (A) The inhibitory activity was determined by ELISA. Each sample was tested in triplicate, and the data are presented as means ± standard deviations. (B) The inhibitory activity was determined by FN-PAGE. The peptide N36 was incubated with HP-OVA at 37°C for 30 min before addition of the peptide C34-FITC at graded concentrations (15, 7.5, 3.75, and 1.875 μM for lanes 3 to 6, respectively). After incubation for another 30 min, the mixtures were analyzed by FN-PAGE.
