TABLE 1.
Effects of concentration of HP in the reaction system on the percentage of modified residues and anti-HIV-1 activity of HP-OVAa
| Concn of HP (mM) | % of modified residuesb |
Anti-HIV-1IIIB activity |
Anti-HIV-1BaL activity |
|||
|---|---|---|---|---|---|---|
| Lysine | Arginine | IC50 (μM) | IC90 (μM) | IC50 (μM) | IC90 (μM) | |
| 2.5 | 63.07 | 43.93 | 3.570 ± 1.170 | >4.48 | >4.48 | >4.48 |
| 5.0 | 89.52 | 58.63 | 0.251 ± 0.073 | 1.033 ± 0.160 | 0.207 ± 0.034 | 2.618 ± 1.946 |
| 10.0 | 96.44 | 59.12 | 0.099 ± 0.025 | 0.364 ± 0.100 | 0.106 ± 0.053 | 0.988 ± 0.836 |
| 20.0 | 98.86 | 71.12 | 0.031 ± 0.001 | 0.095 ± 0.025 | 0.019 ± 0.003 | 0.231 ± 0.034 |
| 40.0 | 99.86 | 89.26 | 0.008 ± 0.000 | 0.027 ± 0.003 | 0.007 ± 0.001 | 0.044 ± 0.019 |
| 60.0 | 99.32 | 92.36 | 0.006 ± 0.001 | 0.019 ± 0.005 | 0.006 ± 0.002 | 0.043 ± 0.026 |
a
Each sample was tested in triplicate, and each experiment was repeated twice. IC50 and IC90 data are presented as means ± standard deviations.
b
The data are averages from two assays.