TABLE 2.
Effects of pH value of the reaction system on the percentage of modified residues and anti-HIV-1 activity of HP-OVAa
| pH value of reaction system | % of modified residuesb |
Anti-HIV-1IIIB activity |
Anti-HIV-1BaL activity |
|||
|---|---|---|---|---|---|---|
| Lysine | Arginine | IC50 (μM) | IC90 (μM) | IC50 (μM) | IC90 (μM) | |
| 3.0 | 0.00 | 22.72 | >4.48 | >4.48 | >4.48 | >4.48 |
| 4.0 | 13.41 | 47.98 | >4.48 | >4.48 | >4.48 | >4.48 |
| 5.0 | 51.16 | 62.11 | 0.352 ± 0.040 | 1.187 ± 0.160 | 0.292 ± 0.060 | >4.48 |
| 6.0 | 96.35 | 78.78 | 0.074 ± 0.026 | 0.208 ± 0.033 | 0.066 ± 0.022 | 1.712 ± 0.969 |
| 7.0 | 98.61 | 79.67 | 0.013 ± 0.002 | 0.036 ± 0.007 | 0.016 ± 0.003 | 0.139 ± 0.134 |
| 8.0 | 98.09 | 78.78 | 0.010 ± 0.002 | 0.025 ± 0.003 | 0.014 ± 0.001 | 0.069 ± 0.005 |
| 9.0 | 98.42 | 89.76 | 0.009 ± 0.001 | 0.029 ± 0.011 | 0.008 ± 0.002 | 0.044 ± 0.009 |
| 10.0 | 98.27 | 89.18 | 0.010 ± 0.001 | 0.023 ± 0.000 | 0.009 ± 0.000 | 0.045 ± 0.005 |
a
Two independent experiments were performed in triplicate. IC50 and IC90 data are presented as means ± standard deviations.
b
The data are averages from two assays.