TABLE 3.
Inhibitory activities of HP-OVA on infection by laboratory-adapted and primary HIV-1 strains and HIV-2, SHIV, and SIV strainsa
| Virus strain | HP-OVA |
T20b |
||
|---|---|---|---|---|
| IC50 (μM) | IC90 (μM) | IC50 (μM) | IC90 (μM) | |
| Laboratory-adapted HIV-1 strains | ||||
| IIIB (clade B, X4) | 0.008 ± 0.000 | 0.027 ± 0.003 | 0.097 ± 0.005 | 0.293 ± 0.042 |
| MN (clade B, X4) | 0.019 ± 0.006 | 0.077 ± 0.030 | 0.047 ± 0.008 | 0.148 ± 0.025 |
| RF (clade B, X4R5) | 0.009 ± 0.001 | 0.026 ± 0.003 | 0.074 ± 0.008 | 0.263 ± 0.122 |
| BaL (clade B, R5) | 0.007 ± 0.001 | 0.044 ± 0.019 | 0.097 ± 0.044 | 0.209 ± 0.077 |
| Primary HIV-1 strains | ||||
| UG94103 (clade A, X4R5) | 0.108 ± 0.031 | 0.289 ± 0.046 | 0.023 ± 0.003 | 0.069 ± 0.015 |
| 92US657 (clade B, R5) | 0.067 ± 0.022 | 1.826 ± 1.440 | 0.120 ± 0.032 | 0.210 ± 0.052 |
| 93IN101 (clade C, R5) | 0.044 ± 0.013 | 0.226 ± 0.200 | NDd | ND |
| 92TH009 (clade A/E, R5) | 0.011 ± 0.004 | 0.032 ± 0.007 | 0.097 ± 0.014 | 0.135 ± 0.008 |
| BR93020 (clade F, X4R5) | 0.376 ± 0.210 | 12.596 ± 18.198 | ND | ND |
| Ru570 (clade G, R5) | 0.578 ± 0.168 | 6.247 ± 2.549 | 0.127 ± 0.010 | 0.236 ± 0.017 |
| BCF02 (clade O, R5) | 0.529 ± 0.201 | 4.131 ± 4.057 | ND | ND |
| Drug-resistant HIV-1 strainsc | ||||
| AZT-resistant strain | 0.072 ± 0.012 | 0.192 ± 0.030 | 0.056 ± 0.008 | 0.116 ± 0.005 |
| NL4-3D36G | 0.063 ± 0.018 | 0.450 ± 0.030 | 0.036 ± 0.020 | 0.312 ± 0.112 |
| NL4-3(36G)V38A | 0.053 ± 0.025 | 0.443 ± 0.054 | >2.000 | >2.000 |
| NL4-3(36G)V38E/N42T | 0.081 ± 0.014 | 0.498 ± 0.170 | >2.000 | >2.000 |
| NL4-3(36G)V38E/N42S | 0.001 ± 0.000 | 0.003 ± 0.000 | >2.000 | >2.000 |
| HIV-2ROD | 1.420 ± 0.190 | 4.751 ± 0.650 | 1.991 ± 0.184 | 10.94 ± 0.507 |
| SHIV | ||||
| SF33A | 0.033 ± 0.008 | 0.188 ± 0.023 | 0.541 ± 0.149 | 10.99 ± 3.779 |
| SF162P3 | 0.450 ± 0.110 | 2.778 ± 0.420 | 0.205 ± 0.109 | 21.12 ± 10.39 |
| SIVmac251 32H | 0.083 ± 0.007 | 1.985 ± 0.320 | 3.051 ± 0.677 | 13.01 ± 3.916 |
a
The measurements were performed in triplicate, and each experiment was repeated at least twice. Data are presented as means ± standard deviations.
b
T20 was used as a control.
c
NL4-3D36G is a T20-sensitive strain which was the parent strain used for the generation of the T20-resistant mutants NL4-3(36G)V38A, NL4-3(36G)V38E/N42T, and NL4-3(36G)V38E/N42S. NL4-3(36G)V38E/N42S is also resistant to T1249 (9).
d
ND, not done.