FIG. 1.

Neighbor-joining tree for the parC QRDR. The Streptococcus agalactiae type strain NCTC 8181 parC sequence (GenBank accession number AB101464) was used to root the tree. If the percentage of replicate trees in which the associated sequences clustered together in the bootstrap test (1,000 replicates) was greater than 75%, the values are shown next to the branches. The tree is drawn to scale, with the branch lengths being in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed by the Kimura two-parameter method and are in the units of the number of base substitutions per site. Sp, Streptococcus pyogenes; Sde, Streptococcus dysgalactiae subsp. equisimilis; Sdd, Streptococcus dysgalactiae subsp. dysgalactiae. Sets of numbers and letters identify the GenBank entries whose sequences are identical to the sequences of the alleles found in our collection or the sequences of S. pyogenes used to construct the tree.