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. 2010 Mar 1;54(5):2219–2224. doi: 10.1128/AAC.00816-09

TABLE 2.

Susceptibility and mRNA expression among control strains and clinical isolates of P. aeruginosa

Isolate MIC (μg/ml)a
 Relative gene expressionb
IPM MEM PTZ CAZ CTX ATM CIP CAR GEN TOB AMK oprD* ampC mexA mexC mexE mexX
Controls
    M1251 4 8 32 4 128 32 0.5 512 0.5 0.25 1 0.29 1.78 7.28 1.24 2.50 2.70
    R70 16 >32 32 8 256 64 0.5 >512 1 0.5 1 0.05 5.72 5.94 1.72 1.32 7.94
    R20 2 0.5 8 1 4 4 ≤0.12 64 ≤0.25 0.5 ≤0.5 3.27 0.73 0.75 0.77 0.30 1.79
    AHP 0.12 0.25 4 0.5 4 0.5 ≤0.12 32 ≤0.12 ≤0.12 ≤0.5 1.23 0.004 0.12 0.02 0.01 0.02
    Z799/61 0.5 ≤0.06 ≤1 ≤0.12 0.5 ≤0.12 ≤0.12 ≤16 ≤0.12 0.25 1 0.15 0.12 0.12 0.24 0.01 0.08
    2779-con 2 1 >64 32 >256 32 ≤0.12 128 0.5 1 1 5.34 313 1.56 0.99 4.90 3.71
    1405-con D2− 16 8 >64 64 >256 64 0.25 512 2 2 4 0.35 599 6.92 2.62 2.26 1.22
Clinical isolates
    1530c 8 0.5 ≤1 4 32 2 8 64 32 4 32 0.06 1.13 0.65 0.69 1.23 11.6
    1693c 8 2 ≤1 8 128 1 1 128 16 2 1 0.08 0.42 2.60 0.38 0.24 0.29
    17145c 32 4 2 64 128 0.5 4 ≤16 32 16 64 0.0004 8.40 0.39 0.11 0.33 2.55
    1529c 16 4 2 4 32 0.5 8 8 >32 16 64 0.12 3.29 0.56 0.18 1.18 12.4
    14169c 16 4 8 8 128 64 1 512 32 4 32 0.07 35.9 2.52 1.05 0.42 4.39
    1247c 16 4 4 8 128 4 4 128 >32 8 >64 0.018 36.4 1.81 0.75 0.44 2.96
    1681c 32 8 >64 256 >256 32 2 512 32 16 64 0.006 11.5 4.90 0.31 0.34 5.85
    1352c >32 16 64 64 >256 32 2 512 >32 16 >64 0.004 10.8 1.82 0.49 0.89 6.41
    1333c 32 16 >64 128 >256 >64 2 >512 >32 8 64 0.0007 368 2.66 1.77 9.01 33.9
    1248c 16 16 32 256 >256 >64 4 >512 >32 32 >64 0.012 5.89 4.11 0.70 1.31 11.7
    15110c >32 16 >64 256 >256 >64 2 >512 >32 >32 >64 0.05 474 5.61 1.00 0.7 2.37
    12142c 32 32 >64 256 >256 >64 4 >512 >32 >32 >64 0.0004 262 15.9 3.1 3.7 6.52
    1712c 32 32 >64 >256 >256 >64 4 >512 >32 16 >64 0.015 160 1.83 1.2 2.98 18.9
    1495c >32 >32 >64 >256 >256 >64 4 >512 >64 8 >64 0.06 284 3.49 2.92 6.91 78.3
    1726 4 0.12 ≤1 2 16 0.5 2 ≤16 2 0.5 2 0.25 1.50 1.08 0.21 0.10 1.80
    15107 8 2 32 8 256 2 0.25 128 >32 2 >64 0.03 1.36 1.87 1.06 0.26 10.5
    16008 64 4 ≤1 1 64 0.5 4 ≤16 16 4 32 0.003 0.09 0.56 0.09 0.03 27.1
    13167 4 4 4 64 >256 16 8 >512 32 4 64 1.53 7.22 3.51 0.76 0.4 1.17
    1240 16 8 8 256 256 4 2 128 2 0.5 4 0.08 110 1.30 1 1.82 2.10
    3149 32 8 16 8 64 64 >8 >512 >32 >32 >64 0.007 10.5 4.87 1.35 0.37 0.3
    12102 32 16 16 4 64 16 2 256 4 1 4 0.0006 0.79 2.46 1.51 1.02 11.8
    1253 >32 32 64 8 128 16 2 256 16 4 32 0.008 4.17 1.96 5.2 7.8 13.3
    13120 16 32 16 4 128 16 0.5 256 0.5 0.25 1 0.9 2.04 2.30 0.87 0.4 15.0
    1665 64 >32 >64 256 >256 >64 4 >512 16 32 >64 0.0001 150 5.89 1.65 1.14 0.51
    17112 128 >32 64 64 >256 >64 2 512 1 0.5 1 0.03 44.1 3.12 0.17 0.08 0.11
a

IPM, imipenem; MEM, meropenem; PTZ, piperacillin-tazobactam; CAZ, ceftazidime; CTX, cefotaxime; ATM, aztreonam; CIP, ciprofloxacin; CAR, carbenicillin; GEN, gentamicin; TOB, tobramycin; AMK, amikacin.

b

Increased or decreased gene expression relative to P. aeruginosa PAO1 (which was assigned a value of 1.0) was defined by expression values of ≥2 and ≤0.5, respectively, and is indicated in bold.

c

Isolate belonging to the Liverpool epidemic strain.