TABLE 2.
Isolate | MIC (μg/ml)a |
Relative gene expressionb |
|||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
IPM | MEM | PTZ | CAZ | CTX | ATM | CIP | CAR | GEN | TOB | AMK | oprD* | ampC | mexA | mexC | mexE | mexX | |
Controls | |||||||||||||||||
M1251 | 4 | 8 | 32 | 4 | 128 | 32 | 0.5 | 512 | 0.5 | 0.25 | 1 | 0.29 | 1.78 | 7.28 | 1.24 | 2.50 | 2.70 |
R70 | 16 | >32 | 32 | 8 | 256 | 64 | 0.5 | >512 | 1 | 0.5 | 1 | 0.05 | 5.72 | 5.94 | 1.72 | 1.32 | 7.94 |
R20 | 2 | 0.5 | 8 | 1 | 4 | 4 | ≤0.12 | 64 | ≤0.25 | 0.5 | ≤0.5 | 3.27 | 0.73 | 0.75 | 0.77 | 0.30 | 1.79 |
AHP | 0.12 | 0.25 | 4 | 0.5 | 4 | 0.5 | ≤0.12 | 32 | ≤0.12 | ≤0.12 | ≤0.5 | 1.23 | 0.004 | 0.12 | 0.02 | 0.01 | 0.02 |
Z799/61 | 0.5 | ≤0.06 | ≤1 | ≤0.12 | 0.5 | ≤0.12 | ≤0.12 | ≤16 | ≤0.12 | 0.25 | 1 | 0.15 | 0.12 | 0.12 | 0.24 | 0.01 | 0.08 |
2779-con | 2 | 1 | >64 | 32 | >256 | 32 | ≤0.12 | 128 | 0.5 | 1 | 1 | 5.34 | 313 | 1.56 | 0.99 | 4.90 | 3.71 |
1405-con D2− | 16 | 8 | >64 | 64 | >256 | 64 | 0.25 | 512 | 2 | 2 | 4 | 0.35 | 599 | 6.92 | 2.62 | 2.26 | 1.22 |
Clinical isolates | |||||||||||||||||
1530c | 8 | 0.5 | ≤1 | 4 | 32 | 2 | 8 | 64 | 32 | 4 | 32 | 0.06 | 1.13 | 0.65 | 0.69 | 1.23 | 11.6 |
1693c | 8 | 2 | ≤1 | 8 | 128 | 1 | 1 | 128 | 16 | 2 | 1 | 0.08 | 0.42 | 2.60 | 0.38 | 0.24 | 0.29 |
17145c | 32 | 4 | 2 | 64 | 128 | 0.5 | 4 | ≤16 | 32 | 16 | 64 | 0.0004 | 8.40 | 0.39 | 0.11 | 0.33 | 2.55 |
1529c | 16 | 4 | 2 | 4 | 32 | 0.5 | 8 | 8 | >32 | 16 | 64 | 0.12 | 3.29 | 0.56 | 0.18 | 1.18 | 12.4 |
14169c | 16 | 4 | 8 | 8 | 128 | 64 | 1 | 512 | 32 | 4 | 32 | 0.07 | 35.9 | 2.52 | 1.05 | 0.42 | 4.39 |
1247c | 16 | 4 | 4 | 8 | 128 | 4 | 4 | 128 | >32 | 8 | >64 | 0.018 | 36.4 | 1.81 | 0.75 | 0.44 | 2.96 |
1681c | 32 | 8 | >64 | 256 | >256 | 32 | 2 | 512 | 32 | 16 | 64 | 0.006 | 11.5 | 4.90 | 0.31 | 0.34 | 5.85 |
1352c | >32 | 16 | 64 | 64 | >256 | 32 | 2 | 512 | >32 | 16 | >64 | 0.004 | 10.8 | 1.82 | 0.49 | 0.89 | 6.41 |
1333c | 32 | 16 | >64 | 128 | >256 | >64 | 2 | >512 | >32 | 8 | 64 | 0.0007 | 368 | 2.66 | 1.77 | 9.01 | 33.9 |
1248c | 16 | 16 | 32 | 256 | >256 | >64 | 4 | >512 | >32 | 32 | >64 | 0.012 | 5.89 | 4.11 | 0.70 | 1.31 | 11.7 |
15110c | >32 | 16 | >64 | 256 | >256 | >64 | 2 | >512 | >32 | >32 | >64 | 0.05 | 474 | 5.61 | 1.00 | 0.7 | 2.37 |
12142c | 32 | 32 | >64 | 256 | >256 | >64 | 4 | >512 | >32 | >32 | >64 | 0.0004 | 262 | 15.9 | 3.1 | 3.7 | 6.52 |
1712c | 32 | 32 | >64 | >256 | >256 | >64 | 4 | >512 | >32 | 16 | >64 | 0.015 | 160 | 1.83 | 1.2 | 2.98 | 18.9 |
1495c | >32 | >32 | >64 | >256 | >256 | >64 | 4 | >512 | >64 | 8 | >64 | 0.06 | 284 | 3.49 | 2.92 | 6.91 | 78.3 |
1726 | 4 | 0.12 | ≤1 | 2 | 16 | 0.5 | 2 | ≤16 | 2 | 0.5 | 2 | 0.25 | 1.50 | 1.08 | 0.21 | 0.10 | 1.80 |
15107 | 8 | 2 | 32 | 8 | 256 | 2 | 0.25 | 128 | >32 | 2 | >64 | 0.03 | 1.36 | 1.87 | 1.06 | 0.26 | 10.5 |
16008 | 64 | 4 | ≤1 | 1 | 64 | 0.5 | 4 | ≤16 | 16 | 4 | 32 | 0.003 | 0.09 | 0.56 | 0.09 | 0.03 | 27.1 |
13167 | 4 | 4 | 4 | 64 | >256 | 16 | 8 | >512 | 32 | 4 | 64 | 1.53 | 7.22 | 3.51 | 0.76 | 0.4 | 1.17 |
1240 | 16 | 8 | 8 | 256 | 256 | 4 | 2 | 128 | 2 | 0.5 | 4 | 0.08 | 110 | 1.30 | 1 | 1.82 | 2.10 |
3149 | 32 | 8 | 16 | 8 | 64 | 64 | >8 | >512 | >32 | >32 | >64 | 0.007 | 10.5 | 4.87 | 1.35 | 0.37 | 0.3 |
12102 | 32 | 16 | 16 | 4 | 64 | 16 | 2 | 256 | 4 | 1 | 4 | 0.0006 | 0.79 | 2.46 | 1.51 | 1.02 | 11.8 |
1253 | >32 | 32 | 64 | 8 | 128 | 16 | 2 | 256 | 16 | 4 | 32 | 0.008 | 4.17 | 1.96 | 5.2 | 7.8 | 13.3 |
13120 | 16 | 32 | 16 | 4 | 128 | 16 | 0.5 | 256 | 0.5 | 0.25 | 1 | 0.9 | 2.04 | 2.30 | 0.87 | 0.4 | 15.0 |
1665 | 64 | >32 | >64 | 256 | >256 | >64 | 4 | >512 | 16 | 32 | >64 | 0.0001 | 150 | 5.89 | 1.65 | 1.14 | 0.51 |
17112 | 128 | >32 | 64 | 64 | >256 | >64 | 2 | 512 | 1 | 0.5 | 1 | 0.03 | 44.1 | 3.12 | 0.17 | 0.08 | 0.11 |
IPM, imipenem; MEM, meropenem; PTZ, piperacillin-tazobactam; CAZ, ceftazidime; CTX, cefotaxime; ATM, aztreonam; CIP, ciprofloxacin; CAR, carbenicillin; GEN, gentamicin; TOB, tobramycin; AMK, amikacin.
Increased or decreased gene expression relative to P. aeruginosa PAO1 (which was assigned a value of 1.0) was defined by expression values of ≥2 and ≤0.5, respectively, and is indicated in bold.
Isolate belonging to the Liverpool epidemic strain.