TABLE 3.
MBCs for monomer and dimer forms of the N-terminal deletion peptides
| Peptide | MBC (μM)a |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Pseudomonas aeruginosa PAO1 |
Escherichia coli ATCC 25922 |
Acinetobacter baumannii ATCC 19606 |
Staphylococcus aureus ATCC 25923 |
MRSA J2918 |
Enterococcus faecalis ATCC 700802 |
|||||||
| Monomer | Dimer | Monomer | Dimer | Monomer | Dimer | Monomer | Dimer | Monomer | Dimer | Dimer | Monomer | |
| Defb14-1CV | 0.3 (±0.0) | 0.2 (±0.0) | 0.6 (±0.0) | 0.3 (±0.0) | 0.6 (±0.0) | 0.3 (±0.0) | 1.4 (±0.5) | 0.5 (±0.0) | 1.2 (±0.0) | 0.6 (±0.0) | 1.2 (±0.2) | 0.6 (±0.2) |
| Defb14-1CVΔ(1) | ND | 0.3 (±0.0) | ND | 1.0 (±0.6) | ND | 0.6 (±0.0) | ND | 0.7 (±0.0) | ND | 0.7 (±0.0) | ND | 0.8 (±0.2) |
| Defb14-1CVΔ(1-2) | 0.7 (±0.0) | 0.5 (±0.2) | 3.3 (±0.8) | 0.6 (±0.2) | 1.7 (±0.4) | 0.6 (±0.0) | 1.0 (±0.3) | 0.8 (±0.2) | 2.1 (±0.4) | 0.8 (±0.6) | 4.2 (±0.8) | 1.0 (±0.2) |
| Defb14-1CVΔ(1-5) | 0.7 (±0.0) | 0.6 (±0.1) | 2.5 (±0.0) | 1.1 (±0.4) | ND | 1.0 (±0.2) | 1.4 (±0.0) | 1.1 (±0.4) | 2.5 (±0.0) | 1.7 (±0.8) | ND | 1.25 (±0.0) |
| Defb14-1CVΔ(1-8) | 1.1 (±0.4) | 0.7 (±0.4) | 10 (±0.0) | 2.5 (±0.0) | 2.9 (±1.1) | 1.3 (±0.6) | 1.5 (±0.0) | 1.1 (±0.3) | 2.2 (±0.4) | 1.3 (±0.0) | >5 | 1.9 (±0.6) |
| Defb14-1CVΔ(1-11) | 1.7 (±0.0) | 0.7 (±0.2) | >10 | >5 | 2.5 (±0.0) | 1.0 (±0.2) | 5.9 (±0.5) | 0.9 (±0.3) | >10 | 1.5 (±1.1) | >5 | >5 |
| Defb14-1CVΔ(1-14) | >15.2 | 1.4 (±0.3) | >10 | >5 | >10 | 2.5 (±0.0) | >15.2 | >7.6 | >10 | 6.7 (±3.3) | >10 | >5 |
| Defb14-1CVΔ(1-17) | >16.5 | 2.6 (±1.0) | >10 | >5 | >10 | 3.3 (±0.8) | >16.5 | >8.3 | >10 | >5 | >10 | >5 |
| Defb14-1CVΔ(1-23) | ND | >10 | ND | >10 | ND | >10 | ND | >10 | ND | >10 | ND | >10 |
| Defb14-1CV(18-23) | >83.5 | NA | ND | ND | ND | ND | >83.5 | NA | ND | NA | ND | ND |
a
Loss of bactericidal activity upon peptide N-terminal deletion is bacterial strain dependent. Sequences of peptides are given in Table 1. Peptides are in either the monomer (DTT treated) or dimer form. Values in parentheses are standard errors of the means. Values in boldface indicate that the MBC for the monomeric form is significantly different (P < 0.01) from that of the Defb14-1CV monomer. ND, not done; NA, not applicable.