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. 2010 Feb 22;54(5):1981–1987. doi: 10.1128/AAC.01236-09

FIG. 1.

FIG. 1.

Susceptibility of wild-type, CsA-resistant, and CsA/NIM-resistant cells to CsA and NIM811. Wild-type (DMSOr), CsAr, and CsA/NIMr clones were treated for 48 h with CsA (A) or NIM811 (B). Viral RNA was quantified by qRT-PCR and normalized to total cellular RNA. The susceptibility curves of a representative experiment (A and B) show the level of viral RNA after drug treatment as the percentage of DMSO control cells. The standard deviation of six replicas is included. The experiment has been repeated several times, and the same trend has been observed. The EC50 for CsA was 0.66, 3.39, and >10 μM in DMSOr, CsAr, and CsA/NIMr replicon-containing cells, respectively. The EC50 for NIM811 was 0.21, 0.83, and 3.5 μM in DMSOr, CsAr, and CsA/NIMr replicon-containing cells, respectively.