FIG. 4.
Contribution of NS5A and NS5B mutations to resistance against NIM811. The NS5A gene of the CsA/NIMr clone was engineered into the wild-type replicon, and the NS5B mutation was added by site-directed mutagenesis. NIM811 susceptibility curves were established. The graph shows the results of two experiments with six replicas each, with standard deviations indicated by error bars. The EC50 for NIM811 was 0.23, 0.94, and 1.14 μM in WT, WT + resistant NS5A, and WT + resistant NS5A & NS5B replicon-containing cells, respectively.