TABLE 2.
Effect of temperature on wrwycr uptake by J774A.1 cells
| Temp (°C) | Result for the indicated input total wrwycr concn at the following time of incubationa: |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 5 h |
24 h |
|||||||||||
| 50 μM |
100 μM |
150 μM |
50 μM |
100 μM |
150 μM |
|||||||
| [Pep]b | D/M ratio | [Pep] | D/M ratio | [Pep] | D/M ratio | [Pep] | D/M ratio | [Pep] | D/M ratio | [Pep] | D/M ratio | |
| 37 | 0.9 | 2.1 | 8.0 | 2.6 | 28.9 | 11.0 | 1.4c | 4.3 | 16.9 | 3.1 | 30.7 | 12.5 |
| 4 | 1.2 | 2.3 | 7.7 | 2.7 | 15.8 | 8.8 | 10.1 | 7.4 | 32.9 | 11.5 | 47.7 | 15.5 |
The total peptide concentration is expressed by using the molecular mass of the peptide monomer rather than that of the peptide dimer. D, peptide dimer; M, peptide monomer.
[Pep], measured intracellular peptide concentration (mM).
This concentration of peptide is unusually lower than the average for the same treatments described in Table 1.