TABLE 1.
Bacterial strains and plasmids used in this study
| Group | Genotype or relevant characteristic(s) | Reference or source |
|---|---|---|
| E. coli strains used for mutagenesis | ||
| HdeA100 | GC4468 zdd-239::Tn9 del1738 (Δ39 kb, including marRAB locus) (Chlr) hdeABp::lacZ (Ampr) on λ at λatt site/pMB102 | 42 |
| HdeA100 mutants | HdeA100 mutagenized using EZ-TN5 <R6Kγori/KAN-2> or EZ-TN5 <Kan-2> transposomes; Ampr Chlr Kanr | This study |
| E. coli strains used for multidrug resistance and gene expression experiments | ||
| BW25113 | Wild type; F− λ− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) rph-1 Δ(rhaD-rhaB)568 hsdR514 | CGSC; 3 |
| JW5248 | BW25113 ΔmarR::kan; Kanr | CGSC; 3 |
| CR1000 | BW25113 ΔmarR | This study |
| CR2000 | BW25113 ΔmarRA | This study |
| CR1000/CR2000 inactivation mutants | Kanr | P1 HdeA100 mutant × CR1000/CR2000 |
| Complementation plasmids | Ptac lacIq; low copy number; Ampr | Mobile plasmid collection |
| pNTR-SD (control) | NBRP (E. coli) (NIG, | |
| pNT3-crp | Japan); 43 | |
| pNTR-SD-hns | ||
| pNTR-SD-cyaA | ||
| pNT3-pcnB | ||
| pNTR-SD-acrB | ||
| pNTR-SD-tolC | ||
| pNTR-SD-damX | ||
| Other plasmids | ||
| pCP20 | Plasmid for excision of kan and cat markers by FLP-mediated site-specific recombination; Ampr Chlr | 12 |
| pKD3 | Template for amplifying the cat gene; Chlr | 12 |
| pKD46 | λRed recombinase expression plasmid; Ampr | 12 |
| pMB102 | ori colE1 lacI lacZp::marA; Ampr | 40 |
| pNN608 | Single copy; acrAp-lacZ; Chlr | 30 |