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. 2010 Mar 8;54(5):1793–1799. doi: 10.1128/AAC.01691-09

FIG. 2.

FIG. 2.

Western blot of whole-cell extracts from katG-deficient E. coli strain UM262 transformed with the empty vector, pTrcHis2-TOPO, or recombinant plasmids expressing various KatG mutations as follows: lanes 1 and 15, WT; lanes 2 and 16, empty vector; lane 3, R463L and D542H; lane 4, S315T and R463L; lane 5, Q127E and R463L; lane 6, P232S and R463L; lane 7, G123E, G299S, and R463L; lane 8, frame shift mutation from position 160; lane 9, S315T and D387H; lane 10, R463L and R489S; lane 11, S315R; lane 12, M420T and R463L; lane 13, A65A, M176T, and R463L; lane 14, H97R and R463L; lane 17, Δ(191W-192E) and R463L; lane 18, N133T; lane 19, R463L; lane 20, R463L and R632C; lane 21, S315T; lane 22, D419H and R463L; lane 23, S383P and R463L; lane 24, frame shift mutation from position 124; lane 25, in-frame insertion and deletion and R463L. The positions of molecular mass markers are shown on the left.