TABLE 3.
Fluorescent neuraminidase inhibition assay of A/Texas/12/2007 (H3N2) virus and plaque-purified clones
Strain designation | Passage levelb | NA mutation | Mean IC50±SD (nM)a |
|||
---|---|---|---|---|---|---|
Oseltamivir | Zanamivir | Peramivir | A-315675 | |||
A/Texas/12/2007 | X/C3 | E119(V/I) | 154.00 ± 3.90 | 3.80 ± 0.27 | 33.56 ± 12.39 | 3.00 ± 1.48 |
A/Texas/12/2007 clone 1 | X/C4 | E119V | 245.50 ± 23.98 | 5.49 ± 0.32 | 1.47 ± 0.02 | 5.45 ± 2.76 |
A/Texas/12/2007 clone 2 | X/C4 | E119I | 430.59 ± 60.51 | 18.59 ± 5.80 | 214.07 ± 10.71 | 23.94 ± 1.39 |
A/Washington/01/2007 (sensitive control) | None | 0.44 ± 0.03 | 1.60 ± 0.05 | 0.50 ± 0.01 | 1.22 ± 0.01 |
Average IC50 of two independent fluorescent NI assays; IC50s of test viruses are compared with those of oseltamivir-sensitive control A/Washington/01/2007 (H3N2) to determine the fold difference in IC50.
X denotes that the virus isolate was grown in the originating laboratory with an unknown passage number and cell culture, and C3 and C4 denote that the same virus isolate was passaged an additional three or four times, respectively, in MDCK cells at the CDC reference laboratory.