TABLE 3.
Phenotypic traits of the wild type (N2), the ugd knockout mutant (dU2), the Ugd-complemented strain (dU2c), the galU knockout mutant (dG1), and the GalU-complemented strain (dG1c)
| Trait | N2 | dU2 | dU2c | dG1 | dG1c |
|---|---|---|---|---|---|
| SDS MIC (μg/ml) | 2,000 | 500 | 2,000 | 500 | 2,000 |
| Relative DNA uptake abilitya | 1 | 3 ± 0.3 | 1 ± 0.2 | 4.9 ± 0.8 | 1.2 ± 0.2 |
| Total LPS (mg/ml) | 13.2 ± 1.2 | 5.0 ± 0.5 | 14.0 ± 0.9 | 4.8 ± 0.4 | 13.7 ± 0.9 |
| Relative invasion abilityb | 1 | 0.2 ± 0.04 | 0.9 ± 0.09 | 0.3 ± 0.05 | 0.8 ± 0.04 |
| AFMc | |||||
| Valley to peak (nm) | 186.70 ± 51.17 | 362.17 ± 127.74 | ND | 262.70 ± 48.94 | ND |
| Roughness (nm) | 182.82 ± 55.13 | 343.23 ± 120.99 | ND | 262.65 ± 49.84 | ND |
a
Relative ratio of DNA uptake ability. The DNA uptake ability of the wild type was set at 1.
b
Relative ratio of cell invasion ability. The cell invasion ability of the wild type was set at 1.
c
At least five cells in three 1-μm2 areas from three independent experiments; each result is expressed as the mean and the standard deviation.