FIG. 2.

Selection experiments with JFH-1. Huh7.5 cells were transfected with RNA-encoding JFH-1wt, and the cells were passaged in the presence of 500 nM or 4,000 nM TMC435 or in the absence of inhibitor (control). Cells were harvested at each passage, and the JFH-1 RNA was quantified by using quantitative RT-PCR and normalized against the quantity of the host RPL13A gene. The JFH-1 RPL13A levels 3 days after transfection (after the first passage and at the start of TMC435 treatment) were set equal to 100%, and the changes over time are shown. White diamonds, cells cultured in the absence of inhibitor; gray squares, cells cultured in the presence of 500 nM TMC435; black triangles, cells cultured in the presence of 4,000 nM TMC435. Time points at which a CPE was observed and no cells could be harvested are indicated with a dashed line.