FIG. 3.
Structural organization of the regions encompassing the ileS2 genes of plasmids pPR9, pV030-8, pUSA03, and pGO400. The respective plasmid names are shown on the left. Restriction endonuclease cleavage sites are abbreviated as follows: E, EcoRI; H, HindIII. IS257 elements (IS-L and IS-R) flanking the ileS2 gene are represented by solid boxes; the white arrows indicate the direction of IS257 transposase transcription. The ileS2 gene and the predicted ORFs upstream and downstream are represented as arrows with the arrowhead indicating their orientation. A segment of approximately 2 kb on pV030-8 has been omitted for clarity. Truncated ORFs are shown using a dotted outline. The positions of the −35 and −10 sequences for the putative promoters Phybrid and Pout are shown above each plasmid. The spacing between the −35 and −10 promoter sequences is indicated below each putative promoter. A distance scale in kb is given below the figure.