TABLE 1.

Bacterial isolates used in this study

Isolate	Expt(s) performed	CEM-101 MIC range (μg/ml)	Characteristic(s)
S. aureus ATCC 29213	Passaging,a single-step mutation, postantibiotic effect, timed-killing, MBC, synergy	0.06-0.12	Macrolide susceptible
S. aureus NRS384	Passagingb	0.12	USA300-0114
S. aureus 004-573D	Passagingb	0.12	USA300c
S. aureus 117-472D	Passagingb	0.12	USA300c
S. aureus 024-11490A	Passagingb	0.12	USA300c
S. aureus 117-453D	Passagingb	0.12	USA300c
S. haemolyticus 064-4090A	Passaginga	0.12	ermA
S. epidermidis 095-2777A	Time-kill, MBC	0.12	Macrolide susceptible
E. faecalis ATCC 29212	Passaging,a single-step mutation, timed-killing	0.03	Macrolide susceptible
E. faecalis 061-6556A	Passagingb	0.06	Erythromycin susceptible
E. faecalis 067-6633A	Passagingb	2	ermB
Enterococcus faecium 067-1457A	Passagingb	0.06	Erythromycin susceptible
E. faecium 086-15387A	Passagingb	1	ermB
S. pneumoniae ATCC 49619	Passaging,a postantibiotic effect, timed-killing, MBC, synergy	0.008-0.015	Macrolide susceptible
S. pneumoniae 063-1085A	Passaging,a single-step mutation, MBC, synergy	0.015	Wild type
S. pneumoniae 075-241B	Passaging,a single-step mutation, timed-killing, MBC	0.015-0.03	ermB
S. pneumoniae 127-2273B	Passaging,a MBC, synergy	0.06	mefA
S. pyogenes 117-1612A	Passaging,a postantibiotic effect, timed-killing, MBC	0.015	Wild type
S. pyogenes 088-11708A	Timed-killing, MBC, synergy	0.06	Macrolide resistant, CEM-101 susceptible
S. mitis 051-4933A	Passaginga	0.12	mefA
S. mitis 112-1885A	Timed-killing, MBC	≤0.008	Macrolide susceptible
H. influenzae ATCC 49247	Postantibiotic effect	2
M. catarrhalis 117-10142A	Postantibiotic effect	0.12	Wild type

^aPassaging with CEM-101, telithromycin, clarithromycin, and azithromycin.

^bPassaging with CEM-101 only.

^cThe pulsed-field gel electrophoresis profile was identical to that of S. aureus strain NRS384 (USA300-0114) from the Network on Antimicrobial Resistance in Staphylococcus aureus.