TABLE 1.

Comparison of results of HRM assay with pyrosequencing and itraconazole susceptibilities of reference A. fumigatus strains^a

Strain	54th codon of cyp51A	Amino acid	HRM genotype	HRM confidence (%)	Pyrosequence	Itraconazolec MIC (μg/ml)
H11-20b	GGG	Gly	GGG	100	GGG	0.5
Af41	GGG	Gly	GGG	98.27	GGG	0.25
RIT12	GAG	Glu	Variation	51.58	GAG	>16
Af72	GAG	Glu	Variation	69.66	GAG	>16
RIT18	GAA	Glu	Variation	24.08	GAA	>16
RIT32	GAA	Glu	Variation	19.82	GAA	>16
RIT15	AAG	Lys	Variation	8.24	AAG	>16
RIT38	AAG	Lys	Variation	8.11	AAG	>16
RIT41	AGG	Arg	Variation	53.22	AGG	>16
RIT51	AGG	Arg	Variation	50.59	AGG	>16
SO/3827A	GTG	Val	Variation	65.0	GTG	>16
SO/3829	GTG	Val	Variation	75.98	GTG	>16
R7-1	TGG	Trp	Variation	41.11	TGG	>16

^aCharacterized strains including itraconazole MIC data were obtained from Balashov et al. (1).

^bUsed as GGG (wild-type) control for HRM assay. The HRM data of all other strains were compared to those of this wild-type control for identification as GGG (wild type) at ≥90% confidence or as a variation (mutant) if <90% confidence.

^cPosaconazole MICs were not determined. Itraconazole and posaconazole are structurally similar. Resistance to itraconazaole is usually associated with a reduction in posaconazole susceptibility (13, 26, 34).