TABLE 1.
Description of the ketoconazole-responsive proteins identified by MALDI-TOF mass spectroscopy
| Protein | CGDb systematic name | Description | pI | Mr (103) | Z score | Protein coverage (%) | No. of matched peptides | Fold change in expressionc | t test |
|---|---|---|---|---|---|---|---|---|---|
| Aco1p | Orf19.6385 | Aconitate hydrotase | 6.0 | 84.2 | 1.7 | 21 | 33 | 2.5 ± 1.4 | 0.02 |
| Acs2p | Orf19.1064 | Acetyl-CoA synthetase | 5.7 | 73.8 | 1.6 | 11 | 6 | 1.6 ± 0.1 | 0.04 |
| Adh2pa | Orf19.5113 | Aldehyde dehydrogenase I | 6.3 | 36.8 | 2.3 | 26 | 8 | 3.8 ± 0.1 | 0.00 |
| Ado1pa | Orf19.5591 | Adenosine kinase | 5 | 38.2 | 2.4 | 47 | 14 | 2.7 ± 1.1 | 0.04 |
| Ald5p | Orf19.5806 | Aldehyde dehydrogenase | 5.5 | 54.0 | 2.3 | 49 | 16 | 1.9 ± 0.8 | 0.02 |
| Ald5p | Orf19.5806 | Aldehyde dehydrogenase | 5.5 | 54.0 | 2.4 | 30 | 11 | 1.6 ± 0.2 | 0.02 |
| Atp2p | Orf19.5653 | F1F0 ATPase complex, β subunit | 4.9 | 55.7 | 2.4 | 48 | 14 | 1.5 ± 0 | 0.00 |
| Cdc19p | Orf19.3575 | Pyruvate kinase | 6.5 | 55.4 | 2.3 | 26 | 10 | 1.9 ± 0.5 | 0.02 |
| Cit1p | Orf19.4393 | Citrate synthase | 6.3 | 48.7 | 2.4 | 30 | 12 | 2 ± 0.3 | 0.00 |
| Erg6pa | Orf19.1631 | Sterol transmethylase | 5.7 | 43.1 | 2.3 | 24 | 8 | 4.3 ± 0.4 | 0.00 |
| Erg10pa | Orf19.1591 | Acetyl-CoA acetyltransferase | 6.5 | 41.9 | 1.7 | 14 | 5 | 2.4 ± 0.7 | 0.00 |
| Erg13p | Orf19.7312 | 3-Hydroxy-3-methylglutaryl-CoA synthase | 5.7 | 49.7 | 2.4 | 42 | 16 | 1.6 ± 0.2 | 0.03 |
| Gnd1p | Orf19.5024 | 6-Phosphogluconate dehydrogenase | 6.1 | 56.9 | 2.3 | 27 | 11 | 1.6 ± 0.5 | 0.10 |
| Idh2p | Orf19.5791 | Isocitrate dehydrogenase | 6.4 | 39.3 | 2.3 | 45 | 12 | 1.5 ± 0 | 0.00 |
| Ino1p | Orf19.7585 | Myoinositol-1-phosphate synthase | 5.3 | 57.8 | 2.3 | 44 | 15 | 2.6 ± 0.4 | 0.00 |
| Csh1pa | Orf19.4477 | Putative aryl-alcohol dehydrogenase | 6.0 | 38.3 | 2.0 | 31 | 10 | 2.4 ± 0.9 | 0.08 |
| Svf1p | Orf19.6068 | Unknown function | 4.9 | 42.9 | 2.3 | 20 | 7 | 1.6 ± 0.2 | 0.02 |
| Ipf17186p | Orf19.251 | Heat shock protein 31 of DJ-1/Pfpl family | 4.7 | 25.8 | 2.3 | 30 | 7 | 1.9 ± 0.1 | 0.00 |
| Ipf17186p | Orf19.251 | Heat shock protein 31 of DJ-1/Pfpl family | 4.7 | 25.8 | 2.2 | 21 | 7 | 3.1 ± 0.3 | 0.00 |
| Lsp1p | Orf19.3149 | Unknown function | 4.9 | 35.6 | 1.4 | 33 | 7 | 2.2 ± 0.2 | 0.02 |
| Meu1p | Orf19.6938 | Regulator of ADH2 expression | 7.2 | 37.6 | 1.9 | 15 | 5 | 2.9 ± 0.7 | 0.02 |
| Mdh11p | Orf19.7481 | Malate dehydrogenase | 5.4 | 36.0 | 1.5 | 24 | 5 | 3.2 ± 1.0 | 0.01 |
| Mdh1pa | Orf19.7481 | Mitochondrial malate dehydrogenase precursor | 5.7 | 34.7 | 2.3 | 37 | 8 | 1.6 ± 0.3 | 0.03 |
| Mge1p | Orf19.2524 | Heat shock protein | 5.6 | 27.2 | 2.4 | 31 | 9 | 3.8 ± 0.1 | 0.00 |
| Pdb1p | Orf19.5294 | Pyruvate dehydrogenase | 5.4 | 41.3 | 2.3 | 30 | 8 | 1.7 ± 0.1 | 0.01 |
| Pfy1p | Orf19.5076 | Actin-binding protein | 5.3 | 13.8 | 2.4 | 51 | 6 | 1.8 ± 0.2 | 0.02 |
| Pgm2p | Orf19.2841 | Phosphoglucomutase | 5.3 | 61.8 | 2.4 | 29 | 13 | 1.9 ± 0.5 | 0.06 |
| Rpl12p | Orf19.1635 | Ribosomal protein | 9.5 | 17.7 | 2.0 | 30 | 6 | 1.7 ± 0.1 | 0.03 |
| Sec1p | Orf19.13833 | Transport protein | 8.1 | 89.6 | 1.3 | 17 | 8 | 2.6 ± 0.5 | 0.03 |
| Snz1p | Orf19.2947 | Stationary-phase protein | 5.8 | 31.8 | 1.7 | 15 | 6 | 1.5 ± 0 | 0.00 |
| Tif5p | Orf19.4261 | Translation initiation factor (eIF-5A) | 5.0 | 16.4 | 1.9 | 33 | 5 | 1.6 ± 0.4 | 0.08 |
| Tuf1pa | Orf19.6047 | Translation elongation factor TU | 5.7 | 46.7 | 2.0 | 24 | 7 | 1.5 ± 0.2 | 0.03 |
| Bat22p | Orf19.6994 | Branched amino acid aminotransferase | 5.9 | 40.8 | 2.1 | 27 | 10 | 0.4 ± 0 | 0.01 |
| Gdh3p | Orf19.4716 | NADP-glutamate dehydrogenase | 5.7 | 49.6 | 2.4 | 23 | 9 | 0.3 ± 0 | 0.01 |
| Gpm1p | Orf19.903 | Phosphoglycerate mutase | 5.8 | 27.4 | 2.3 | 40 | 7 | 0.4 ± 0 | 0.00 |
| Hsp90p | Orf19.6515 | Heat shock protein | 4.8 | 80.8 | 1.8 | 21 | 14 | 0.3 ± 0.1 | 0.05 |
| Pdc11p | Orf19.2877 | Pyruvate decarboxylase | 5.4 | 62.4 | 2.3 | 30 | 14 | 0.5 ± 0.1 | 0.00 |
| Psa1p | Orf19.4943 | GDP-mannose pyrophosphorylase | 5.9 | 40.0 | 2.3 | 25 | 8 | 0.2 ± 0 | 0.00 |
| Ssa1p | Orf19.1065 | Heat shock protein of HSP family | 4.9 | 70.1 | 2.4 | 21 | 12 | 0.5 ± 0.2 | 0.08 |
a
Proteins whose genes were previously shown to be differentially expressed by microarray analysis.
b
CGD, Candida Genome Database.
c
Change in expression between treated cells and control cells.