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. 2010 Mar 8;30(10):2330–2340. doi: 10.1128/MCB.01675-09

FIG. 3.

FIG. 3.

Cytoplasmic signaling by both Gal1p and Gal3p is required for optimal GAL gene reinduction. (a) RT-PCR of wild-type and gal3Δ;Ptet-GAL3 strains showing GAL3 expression levels in glucose or galactose. (b) Minimal expression of Gal3p from a Ptet-GAL3 construct causes a partial defect in GAL1 reinduction following short-term glucose repression (1 h). Shown are GAL1 mRNA levels for the wild-type (left) and gal3Δ-Ptet-GAL3 (right) strains. At top is a representative RT-PCR, and the graphs below show data from an average of three independent experiments represented as fold increases over ACT1 mRNA normalized to a maximum value of 1. Error bars show standard deviations at each point. o/n, overnight. (c) GAL10 is induced in minimally expressing Ptet-GAL3 and an amino-terminal deletion of a GAL1 double mutant but is lost at 2 h postinduction. GAL10 reinduction is severely compromised even after 8 h following short-term glucose repression (1 h). Data in panel c are representative of five independent experiments.