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. 2010 Mar 8;30(10):2485–2497. doi: 10.1128/MCB.01633-09

FIG. 3.

FIG. 3.

Analysis of the effect of the loss and overexpression of dUTX on global levels of H3K27 and H3K4 methylation. (A to D) Eye imaginal discs were labeled with antibodies specific for the indicated histone methyl modifications. GFP fluorescence was used to identify dUTX clones. Global levels of H3K27me2 (A), H3K27me1 (B), H3K4me2 (C), and H3K4me3 (D) in dUTX mutant clones were unchanged. Left panels are the merged GFP and antibody channels. The right panels are the antibody-only channels. (E and F) Wing imaginal discs that overexpress dUTX in the posterior compartment marked by GFP (green) were labeled with antibodies specific for H3K27me3 (E) and H3K4me1 (F). Left panels are the merged GFP and antibody channels. The right panels are the antibody-only channels. Global levels of H3K27me3 and H3K4me1 are unchanged. Similar data were obtained for the catalytic mutant dUTXJmjc* (data not shown). Genotypes are as follows: ey-FLP; dUTX1 FRT40A/P[ubi-GFP] FRT40A (A to D) and en-Gal4 UAS-dUTX UAS-GFP (E and F).