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. 2010 Mar 8;30(10):2485–2497. doi: 10.1128/MCB.01633-09

FIG. 5.

FIG. 5.

dUTX regulates the cell cycle. (A) Quantification of BrdU incorporation of S2 cells without RNAi treatment (S2 control) and with pUC19 RNAi (negative control) and three different double-stranded RNAs (dsRNAs) against dUTX. (B) Assessment of dUTX mRNA knockdown of the three different dUTX dsRNAs used in the BrdU incorporation assay (A) relative to pUC19 RNAi controls. mRNA levels of rp49 (negative control) are unaffected. (C to F) Heterozygosity for E(z) (D) and Pc (E) suppresses the overrepresentation phenotype of dUTX1 mosaics (C). Genotypes are as follows: ey-FLP; dUTX1 FRT40A/P[w+] FRT40A (C), ey-FLP; dUTX1 FRT40A/P[w+] FRT40A; E(z)731/+ (D), and ey-FLP; dUTX1 FRT40A/P[w+] FRT40A; Pc1/+ (E).