FIG. 6.
dUTX interacts genetically with Notch. (A) Change of mRNA levels in dUTX mutant larvae. qRT-PCR analysis of transcript levels of the indicated genes from homozygous mutant dUTX1 and dUTXPB third-instar larvae. The levels are normalized to 100% for wild-type larvae (red line). Note that dUTX mRNA levels in dUTX1 and dUTXPB mutant larvae are also reduced. (B and B′) Anti-Notch antibody labeling of dUTX1 mosaic eye imaginal discs. The posterior is to the right, and the dorsal half is up. (B) Merged GFP and antibody channels. (B′) Antibody-only channel. dUTX clones are marked by the absence of GFP. Clones in the ventral half of the eye disc, especially in the morphogenetic furrow, contain elevated levels of the Notch protein. The anti-Notch antibody was raised against the intracellular domain. (C) Heterozygosity of Notch suppresses the overrepresentation phenotype of dUTX mosaic eyes (compare to Fig. 1B and 4C). (D and E) Wing-notching phenotype caused by the loss of one gene dose of Notch (N) (D) and Serrate (Ser) (E). (D′, D″, E′, E″, and E‴) Heterozygosity of dUTX dominantly suppresses the wing-notching phenotype of N and Ser. The suppression varies from mild (D′ and E′) to complete (D″ and E‴), which is quantified in F and G for the three dUTX alleles. Genotypes are as follows: ey-Flp; dUTX1 FRT40A/P[ubi-GFP] FRT40A (B and B′), ey-FLP/N264-39; dUTX1 FRT40A/P[w+] FRT40A (C), N8/+; P[y+] FRT40A/+ (D), N8/+; dUTX1 FRT40A/+ (D′ and D″), Ser1/+ (E), and dUTX1 FRT40A/+; Ser1/+ (E′ to E‴).