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Purification of CDK8-Mediator complex from HeLa cells. (A) Purification scheme for isolation of human CDK8-Mediator from nuclear extract prepared from HeLa cells stably expressing f:MED17. (B) Silver staining of purified human CDK8-Mediator. (C) Immunoblot analyses of purified CDK8-Mediator complexes isolated directly by using an anti-FLAG affinity column (M2 IP) or purified via P11 phosphocellulose chromatography followed by anti-FLAG affinity purification (CDK8 MED).
