FIG. 6.

Loss of CDK8 expression reduces Pol II occupancy at T3-responsive gene promoters and decreases recruitment of CDK9 at the DioI gene. (A) Schematic representation of the DioI gene, showing the locations of PCR primers in the proximal promoter and downstream intragenic regions. (B, C, and D) α-2 cells were transfected with CDK8 siRNA or a control siRNA for 72 h and then treated with or without T3 (10⁻⁷ M) for 1 h. The cells were then processed for ChIP analyses using the antibodies shown to the right of the panels and specific PCR primer sets indicated below the panels. (E and F) ChIP was carried out exactly as described above except that PCR primers specific for the T3-responsive FAS and ADRB2 gene promoters were used.