FIG. 7.

CDK8 kinase activity is required for full T3-dependent DioI mRNA expression. α-2 cells were transfected with either scrambled or CDK8-specific siRNAs along with either a wild-type CDK8 expression vector or a mutant CDK8 expression vector (D151A) deficient in kinase activity. At 72 h posttransfection, the cells were treated with or without T3 for 1 h and then harvested. Total RNA was processed by quantitative RT-PCR in real time using primers specific for DioI, while whole-cell lysate was analyzed by immunoblotting using antibodies specific for CDK8 and α-tubulin.