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. 2010 Mar 22;30(10):2449–2459. doi: 10.1128/MCB.01604-09

FIG. 7.

FIG. 7.

Depletion of ABH8 diminishes cellular survival after exposure to DNA-damaging agents. (A) Colony formation assay of human cell lines after treatment with the indicated doses of MMS. (B and C) Viability of control and ABH8-depleted human cell lines after treatment with the indicated doses of MMS or bleomycin as measured by trypan blue dye exclusion or by the WST-1 cell proliferation and viability assay. (D) Verification of full-length ABH8 expression in human cell lines. Whole-cell extracts prepared from the indicated human cell lines were immunoblotted with antibody probes against the respective proteins. The asterisk denotes a protein that cross-reacts with the anti-ABH8 antibody. (E and F) Viability of human cell lines after treatment with MMS or bleomycin. (G) Real-time RT-PCR analysis of ABH8 transcripts from the indicated human cells. (H) Confirmation of URM1 depletion in human cells. (I) Viability of control and URM1-depleted human cell lines after treatment with MMS.