FIG. 1.

Changes in histone acetylation levels at group D/E gene promoters in cells depleted of dREAM/MMB components. (A) Western blot analysis of whole-cell extracts from SL2 cells incubated with double-stranded RNA (dsRNA) targeting dE2F2, p55CAF1, or white (control). β-Tubulin served as loading control. (B) Northern blot analysis of total RNA extracted from cells incubated with dsRNA targeting Mip40, Mip120, or white. (C and D) ChIP assay was performed with antibodies recognizing panacetylated histone H3 (left panels) or panacetylated histone H4 (right panels) in cells incubated with dsRNA targeting white (control), dE2F2, p55CAF1, Mip40, or Mip120. The amount of coprecipitated DNA was determined by quantitative real-time PCR. Results are normalized to those for a nonspecific sequence (promoter of RP49) and represent the averages of the results of three independent experiments. The promoter region of CG7628 (a non-E2F-regulated gene) and sequences surrounding the regulatory region of the bithoraxoid gene (bxdPRE) were used as negative controls.