FIG. 8.

Runx1 addition to Runx1 cKO keratinocytes rescues their proliferation defect. (A) RT-PCR analysis performed in triplicate with Runx1, -2, -3 and GAPDH primer pairs as indicated. cDNA was prepared from three cell types (indicated on the right in the upper panel and at the top of the lower panel). L, DNA ladder. (B and C) RT-PCR (B) and QRT-PCR (C) analyses of mRNA levels in freshly sorted bulge (CD34⁺) and nonbulge (CD34⁻) cells. WT mice showed weak and variable expression in mice from different litters, while iKO mice showed little if any differences from WT littermates. (D) Quantification of BrdU⁺ cells in primary keratinocytes isolated from WT and Runx1 cKO cells transfected with the constructs schematized at the top.