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Forced expression of Runx2 increases estrogen production in bone cells. The concentration of estrogen in culture medium was measured after infection with adenovirus expressing Runx2 in two bone cell lines, HOS and MG63. The forced expression of Runx2 increased estrogen production in both HOS (A) and MG63 (B) cell lines. Ad, adenovirus. The values represent the means ± SD from three experiments. *, P < 0.05; **, P < 0.005.
