FIG. 4.

LS fibroblasts display enhanced EGF-induced GSK-3β phosphorylation. (A) (Top) Immortalized WT and LS (T468M or Y279C mutation) fibroblasts were stimulated with EGF and then processed for Western blot analysis and probed with anti-phospho-GSK-3β (P-GSK-3β), anti-total GSK-3β, and antitubulin antibodies. (Bottom) P-GSK-3β immunoblots were quantified using ImageJ. Only significant differences versus WT cells for the corresponding time are indicated (*, P < 0.05; ***, P < 0.001; n = 3). The error bars indicate SEM. (B) The same experiment as shown in panel A with cells from WT or NS patients carrying the indicated PTPN11 mutations.