FIG. 6.

LS mutations promote upregulation of SRF activity through a PI3K-dependent pathway. (A) SRF activity was measured using a luciferase gene reporter assay in LS (T468M), NS (N308D), and control (WT) fibroblasts in the presence of increasing concentrations of myocardin. The error bars indicate SEM. (B) The same experiment as shown in panel A performed with all available patient cell lines. Significant differences versus stimulated WT cells are indicated (*, P < 0.05; ***, P < 0.001; n.s., not significant). (C) The same experiment as shown in panel A performed in the presence of the indicated inhibitors. **, P < 0.01; n.s., not significant. Significant differences versus corresponding cells, stimulated with EGF in the absence of inhibitor, are indicated.