FIG. 7.

Effects of spt5-CTD mutations in combination with rpb1-S2A. The amino acid sequence of the S. pombe Rpb1 CTD-S2A variant is shown at right. The heptapeptide repeats are stacked vertically with the mutated Ser2 positions shaded in gray. The indicated fission yeast strains were maintained in logarithmic growth at 30°C in YES medium. The cultures were adjusted to an A₆₀₀ of 0.01, and aliquots of 10-fold serial dilutions were spotted on YES agar medium. The genotypes of the strains with respect to the rpb1 and spt5 loci are indicated on the left. The plates shown in the upper panels were incubated for either 2 days at 37 and 30°C, 3 days at 25°C, or 5 days at 20°C. The plates shown in the lower panels were incubated for either 2 days at 37 and 30°C, 4 days at 25°C, or 7 days at 20°C.