FIG. 6.

Immunofluorescence analysis of the intracellular expression of μNS-Mi chimeras. CEFs were transfected with plasmids expressing μNS-Mi (a1) or the different constructs indicated above each panel. The four domains present in μNS-Mi are represented following the scheme shown in Fig. 5. The green barrel represents green fluorescent protein, whereas blue and yellow truncated cones represent CA-C and CA-C-M, respectively. The transfected cells were stained as in Fig. 2b, except those containing GFP that were visualized without antibodies. (a) Substitution of Coil1. (b) Substitution of C-Tail.