FIG. 6.

Correlation of HA binding, ζ chain expression, and NK cell cytotoxicity. Primary NK cells were treated with HA at the indicated concentrations for 24 h. The cells were collected and examined for the presence of surface and intracellular HA (A) and for the intracellular expression of the ζ chain (B). The treated NK cells also were determined for their redirected cytotoxicity against P815 cells in the presence of anti-NKp46 antibody at an E/T ratio of 30:1. (C) Samples were analyzed by flow cytometry for the percentage of specific lysis. The data shown are means ± SEM from four different donors. *, P < 0.05; ***, P < 0.001.