FIG. 3.

CD4 and chimeric constructs containing TMD and mutant TMD of DENV2, surface expression, and glycosylation pattern. (A) Schematic diagram of the CD4 construct (pCB-CD4) and chimeric CD4 constructs containing TMD and mutant TMD of DENV2. (B) 293T cells transfected with each of these constructs were subjected to flow cytometry analysis as described for Fig. 1C. Relative surface expression (means and standard errors) from three experiments is shown. (C) Lysates derived from 293T cells transfected with the constructs described above were treated with endo H (H) or PNGase F (F) and subjected to Western blot analysis by using MAb RPA-T4. Data were presented as described in the legend to Fig. 1D. (D) The ratio of the intensity of the endo H-resistant band to that of the endo H-sensitive band was determined for each construct. Data are means and standard errors of three experiments.