FIG. 7.

Production of VLPs by PrM/E expression constructs containing different mutations in the TMD (T1 and T2 helices). Forty-eight hours after transfection to 293T cells, cell lysates (A) and pellets (B) derived from culture supernatants by 20% sucrose cushion ultracentrifugation were subjected to Western blot analysis using serum from a confirmed DENV2 case (70). Arrowheads indicate PrM and E proteins recognized by the DENV2 serum, which did not react with lysates of mock-transfected cells (data not shown). The PrM bands of pellets after long exposure are shown below. Molecular mass markers are shown in kDa. One representative experiment of more than three is shown.