TABLE 2.

Comparison of the length and number of nonhydrophobic residues of TMD of E proteins of viruses assembled at different sites^a

Virusb	Site of assemblyc	Length of TMD	No. of nonhydrophobic residues in TMD	Reference(s)
HIV-1	PM	22	5	18, 30
Influenza A virus	PM	27	8	7, 30, 63
Ebola virus	PM	24	4	20, 30, 42
Sindbis virus	PM	26	5	16, 25, 30
Bunyaviridae
UUKV	Golgi	19-20	3-4	2, 15, 33, 49, 66
RVFV	Golgi	20	2
ANDV	Golgi	19	4
BUNV	Golgi	19	2
La Crosse virus	Golgi	19	4
Coronavirus	ER-Golgi intermediate	E, 17	E, 6	30, 36, 67
DENV1	ER	T1, 16; T2, 18	T1, 7; T2, 7; T1-T2, 5	Predicted from reference 74
DENV2	ER	T1, 16; T2, 18	T1, 6; T2, 7; T1-T2, 5	74
DENV3	ER	T1, 16; T2, 18	T1, 6; T2, 7; T1-T2, 5	Predicted from reference 74
DENV4	ER	T1, 16; T2, 18	T1, 4; T2, 6; T1-T2, 6	Predicted from reference 74
JEV	ER	T1, 16; T2, 18	T1, 6; T2, 5; T1-T2, 4	68; predicted from reference 74
YFV	ER	T1, 16; T2, 18	T1, 5; T2, 5; T1-T2, 4	31; predicted from reference 74
WNV	ER	T1, 16; T2, 18	T1, 6; T2, 5; T1-T2, 4	40
TBEV	ER	T1, 16; T2, 18	T1, 4; T2, 6; T1-T2, 3	Predicted from reference 74

^aThe TMD of flaviviruses consists of two α-helices (T1 and T2) defined based on a recent cryo-EM study (74) and a stretch of six mostly nonhydrophobic residues between them (T1-T2). For coronavirus, only the E protein, which is the major determinant of localization in the ER-Golgi intermediate, is analyzed. Similarly, for bunyaviridae, only the Gn protein, the major determinant of localization in the Golgi complex, is analyzed.

^bHIV-1, human immunodeficiency virus type 1; UUKV, Uukuniemi virus; RVFV, Rift Valley fever virus; ANDV, Andes virus; BUNV, Bunyamwera virus; DENV1, DENV2, DENV3, and DENV4: dengue virus type 1, 2, 3 and 4, respectively; JEV, Japanese encephalitis virus; YFV, yellow fever virus; WNV, West Nile virus; TBEV, tick-borne encephalitis virus.

^cPM, plasma membrane; ER, endoplasmic reticulum.