FIG. 3.

att and ts mutations affect viral protein expression of Sw99-att and Sw99-ts at different temperatures. (A) Western blot against NS1 protein. MDCK cells were infected with the different viruses at an MOI of 2. Cells were harvested at different points p.i. Whole-cell lysates were subjected to Western blot analysis using anti-1-73NS1TX/98 (28). (B) Coom., levels of NP protein determined by immunoprecipitation and Coomassie blue staining. MDCK cells were infected with the different viruses at an MOI of 2. Cells were harvested at 5 h p.i. NP protein from whole-cell lysates was immunoprecipitated using anti-PR8 antibody. NP was visualized by Coomassie blue staining. Auto., levels of NP determined by immunoprecipitation in metabolic-labeled cells. MDCK cells were labeled for 5 h in the presence of ³⁵S-Met-Cys. NP protein from whole-cell lysates was immunoprecipitated using anti-PR8 antibody. NP was visualized by autoradiography. HC, heavy chain.