FIG. 8.

Fluorescent staining of CVS-infected DRG cultures without NAC treatment (A, C, and E) and with NAC treatment (B, D, and F) with staining for β-tubulin (red) (A and B), 4-HNE (green) (C and D), and a merging (yellow) (E and F). In the NAC-treated culture (D and F), there was a marked reduction in the expression of 4-HNE versus the untreated culture (C and E). Axonal swellings (arrowheads) remained present in both the untreated (E) and NAC-treated (F) cultures, but coexpression of β-tubulin and 4-HNE was much greater in the untreated culture (E).