Skip to main content
. 2010 Feb 24;84(9):4534–4542. doi: 10.1128/JVI.02487-09

FIG. 1.

FIG. 1.

Creation of stable 293T cell clones latently infected with WT, PKmut, and TKmut viruses. (A) 293T cells latently infected with EBV WT or PKmut viruses were transfected with vector control or BZLF1 and BRLF1 expression vectors (“Z”) to induce lytic gene expression, and extracts were analyzed by immunoblotting for EBV-PK, BZLF1, and β-actin. (B) 293T cells latently infected with EBV WT or PKmut viruses were transfected with vector control or BZLF1 and BRLF1 expression vectors in the presence or absence of a cotransfected EBV-PK expression vector, as indicated. Extracts were analyzed by immunoblotting for BZLF1, BMRF1, and β-actin. The hyperphosphorylated (pp-BMRF1) and phosphorylated (p-BMRF1) forms of the BMRF1 protein are indicated; the hyperphosphorylated form of BMRF1 requires expression of EBV-PK (10, 30, 31). (C) 293T cells infected with WT or TKmut viruses were transfected with vector control or the BZLF1 (Z) and BRLF1 expression plasmids. Extracts were prepared and analyzed by immunoblotting for expression of the lytic EBV proteins, BMRF1, BZLF1, EBV-TK, and cellular β-actin.