FIG. 5.

Expression of EBV-PK in trans restores susceptibility of PKmut to ganciclovir and acyclovir. 293T cells infected with WT or PKmut viruses were transfected with BZLF1/BRLF1/gp110 expression vectors to induce lytic replication in the presence or absence of a cotransfected EBV-PK expression vector, as indicated. Following transfection, cells were treated with no drug or GCV (5 μg/ml) (A) or ACV (25 μg/ml) (B) for 48 h, and viral titers were quantitated. The average viral titer produced for each transfection condition in the absence of drug is set as 100% for each cell line (± standard deviation). The average viral titer of cells treated with GCV or ACV is normalized to the average viral titer of cells in the absence of drug.